Duplex molecular assay intended for point-of-care diagnosis of influenza A/B virus infection.
Identifieur interne : 000048 ( Main/Exploration ); précédent : 000047; suivant : 000049Duplex molecular assay intended for point-of-care diagnosis of influenza A/B virus infection.
Auteurs : Liang-Ta Wu [Royaume-Uni] ; Isabelle Thomas ; Martin D. Curran ; Joanna S. Ellis ; Surendra Parmar ; Neha Goel ; Pia I. Sharma ; Jean-Pierre Allain ; Helen H. LeeSource :
- Journal of clinical microbiology [ 1098-660X ] ; 2013.
Descripteurs français
- KwdFr :
- Belgique, Grippe humaine (diagnostic), Grippe humaine (virologie), Humains, Muqueuse nasale (virologie), Partie nasale du pharynx (virologie), Pharynx (virologie), Royaume-Uni, Sensibilité et spécificité, Systèmes automatisés lit malade, Techniques de diagnostic moléculaire (), Virologie (), Virus de la grippe A (isolement et purification), Virus influenza B (isolement et purification).
- MESH :
- diagnostic : Grippe humaine.
- isolement et purification : Virus de la grippe A, Virus influenza B.
- virologie : Grippe humaine, Muqueuse nasale, Partie nasale du pharynx, Pharynx.
- Belgique, Humains, Royaume-Uni, Sensibilité et spécificité, Systèmes automatisés lit malade, Techniques de diagnostic moléculaire, Virologie.
- Wicri :
- geographic : Belgique, Royaume-Uni.
English descriptors
- KwdEn :
- Belgium, Humans, Influenza A virus (isolation & purification), Influenza B virus (isolation & purification), Influenza, Human (diagnosis), Influenza, Human (virology), Molecular Diagnostic Techniques (methods), Nasal Mucosa (virology), Nasopharynx (virology), Pharynx (virology), Point-of-Care Systems, Sensitivity and Specificity, United Kingdom, Virology (methods).
- MESH :
- geographic : Belgium, United Kingdom.
- diagnosis : Influenza, Human.
- isolation & purification : Influenza A virus, Influenza B virus.
- methods : Molecular Diagnostic Techniques, Virology.
- virology : Influenza, Human, Nasal Mucosa, Nasopharynx, Pharynx.
- Humans, Point-of-Care Systems, Sensitivity and Specificity.
Abstract
Early diagnosis and management of influenza virus infection directly correlates with the effectiveness in disease control. Current molecular influenza virus tests were designed for use in diagnostic testing facilities, where sophisticated equipment and highly trained technicians are available. A longer turnaround time for the centralized testing than when testing near the sample source could delay the initiation of medical intervention, thereby reducing the efficacy of antiviral treatment. The new assay, the SAMBA (simple amplification-based assay) Flu duplex test, is a dipstick-based molecular assay developed to provide a simple, accurate, and cost-effective solution for the diagnosis of influenza A/B viruses intended for near-patient testing. The test presents an alternative format of influenza virus molecular testing that utilizes isothermal amplification and visual detection of nucleic acid on a test strip. The entire test procedure (extraction, amplification, and detection) is integrated into an enclosed semiautomated system. Analytically, the SAMBA Flu duplex test detects 95 and 85 copies of viral genomes for influenza A and B viruses, respectively, with no cross-reactivity observed against other common respiratory pathogens. The clinical performance was established by blind testing of 328 nasal/throat and nasopharyngeal swab specimens from the United Kingdom and Belgium and comparing the results with the quantitative reverse transcription-PCR method routinely used in two public health laboratories. The SAMBA Flu duplex test showed a clinical sensitivity and specificity of 100% and 97.9% for influenza virus A and 100% and 100% for influenza virus B. The test provides a new technology that could facilitate simple and timely identification of influenza virus infection, potentially resulting in more efficient control measures.
DOI: 10.1128/JCM.00740-13
PubMed: 23850955
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Curran</name></author><author><name sortKey="Ellis, Joanna S" sort="Ellis, Joanna S" uniqKey="Ellis J" first="Joanna S" last="Ellis">Joanna S. Ellis</name></author><author><name sortKey="Parmar, Surendra" sort="Parmar, Surendra" uniqKey="Parmar S" first="Surendra" last="Parmar">Surendra Parmar</name></author><author><name sortKey="Goel, Neha" sort="Goel, Neha" uniqKey="Goel N" first="Neha" last="Goel">Neha Goel</name></author><author><name sortKey="Sharma, Pia I" sort="Sharma, Pia I" uniqKey="Sharma P" first="Pia I" last="Sharma">Pia I. Sharma</name></author><author><name sortKey="Allain, Jean Pierre" sort="Allain, Jean Pierre" uniqKey="Allain J" first="Jean-Pierre" last="Allain">Jean-Pierre Allain</name></author><author><name sortKey="Lee, Helen H" sort="Lee, Helen H" uniqKey="Lee H" first="Helen H" last="Lee">Helen H. Lee</name></author></analytic><series><title level="j">Journal of clinical microbiology</title><idno type="eISSN">1098-660X</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Belgium</term><term>Humans</term><term>Influenza A virus (isolation & purification)</term><term>Influenza B virus (isolation & purification)</term><term>Influenza, Human (diagnosis)</term><term>Influenza, Human (virology)</term><term>Molecular Diagnostic Techniques (methods)</term><term>Nasal Mucosa (virology)</term><term>Nasopharynx (virology)</term><term>Pharynx (virology)</term><term>Point-of-Care Systems</term><term>Sensitivity and Specificity</term><term>United Kingdom</term><term>Virology (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Belgique</term><term>Grippe humaine (diagnostic)</term><term>Grippe humaine (virologie)</term><term>Humains</term><term>Muqueuse nasale (virologie)</term><term>Partie nasale du pharynx (virologie)</term><term>Pharynx (virologie)</term><term>Royaume-Uni</term><term>Sensibilité et spécificité</term><term>Systèmes automatisés lit malade</term><term>Techniques de diagnostic moléculaire ()</term><term>Virologie ()</term><term>Virus de la grippe A (isolement et purification)</term><term>Virus influenza B (isolement et purification)</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Belgium</term><term>United Kingdom</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Grippe humaine</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A virus</term><term>Influenza B virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus de la grippe A</term><term>Virus influenza B</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Molecular Diagnostic Techniques</term><term>Virology</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Grippe humaine</term><term>Muqueuse nasale</term><term>Partie nasale du pharynx</term><term>Pharynx</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Influenza, Human</term><term>Nasal Mucosa</term><term>Nasopharynx</term><term>Pharynx</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Point-of-Care Systems</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Belgique</term><term>Humains</term><term>Royaume-Uni</term><term>Sensibilité et spécificité</term><term>Systèmes automatisés lit malade</term><term>Techniques de diagnostic moléculaire</term><term>Virologie</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Belgique</term><term>Royaume-Uni</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Early diagnosis and management of influenza virus infection directly correlates with the effectiveness in disease control. Current molecular influenza virus tests were designed for use in diagnostic testing facilities, where sophisticated equipment and highly trained technicians are available. A longer turnaround time for the centralized testing than when testing near the sample source could delay the initiation of medical intervention, thereby reducing the efficacy of antiviral treatment. The new assay, the SAMBA (simple amplification-based assay) Flu duplex test, is a dipstick-based molecular assay developed to provide a simple, accurate, and cost-effective solution for the diagnosis of influenza A/B viruses intended for near-patient testing. The test presents an alternative format of influenza virus molecular testing that utilizes isothermal amplification and visual detection of nucleic acid on a test strip. The entire test procedure (extraction, amplification, and detection) is integrated into an enclosed semiautomated system. Analytically, the SAMBA Flu duplex test detects 95 and 85 copies of viral genomes for influenza A and B viruses, respectively, with no cross-reactivity observed against other common respiratory pathogens. The clinical performance was established by blind testing of 328 nasal/throat and nasopharyngeal swab specimens from the United Kingdom and Belgium and comparing the results with the quantitative reverse transcription-PCR method routinely used in two public health laboratories. The SAMBA Flu duplex test showed a clinical sensitivity and specificity of 100% and 97.9% for influenza virus A and 100% and 100% for influenza virus B. The test provides a new technology that could facilitate simple and timely identification of influenza virus infection, potentially resulting in more efficient control measures. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23850955</PMID><DateCompleted><Year>2014</Year><Month>03</Month><Day>10</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1098-660X</ISSN><JournalIssue CitedMedium="Internet"><Volume>51</Volume><Issue>9</Issue><PubDate><Year>2013</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Journal of clinical microbiology</Title><ISOAbbreviation>J. Clin. Microbiol.</ISOAbbreviation></Journal><ArticleTitle>Duplex molecular assay intended for point-of-care diagnosis of influenza A/B virus infection.</ArticleTitle><Pagination><MedlinePgn>3031-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1128/JCM.00740-13</ELocationID><Abstract><AbstractText>Early diagnosis and management of influenza virus infection directly correlates with the effectiveness in disease control. Current molecular influenza virus tests were designed for use in diagnostic testing facilities, where sophisticated equipment and highly trained technicians are available. A longer turnaround time for the centralized testing than when testing near the sample source could delay the initiation of medical intervention, thereby reducing the efficacy of antiviral treatment. The new assay, the SAMBA (simple amplification-based assay) Flu duplex test, is a dipstick-based molecular assay developed to provide a simple, accurate, and cost-effective solution for the diagnosis of influenza A/B viruses intended for near-patient testing. The test presents an alternative format of influenza virus molecular testing that utilizes isothermal amplification and visual detection of nucleic acid on a test strip. The entire test procedure (extraction, amplification, and detection) is integrated into an enclosed semiautomated system. Analytically, the SAMBA Flu duplex test detects 95 and 85 copies of viral genomes for influenza A and B viruses, respectively, with no cross-reactivity observed against other common respiratory pathogens. The clinical performance was established by blind testing of 328 nasal/throat and nasopharyngeal swab specimens from the United Kingdom and Belgium and comparing the results with the quantitative reverse transcription-PCR method routinely used in two public health laboratories. The SAMBA Flu duplex test showed a clinical sensitivity and specificity of 100% and 97.9% for influenza virus A and 100% and 100% for influenza virus B. The test provides a new technology that could facilitate simple and timely identification of influenza virus infection, potentially resulting in more efficient control measures. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Liang-Ta</ForeName><Initials>LT</Initials><AffiliationInfo><Affiliation>Diagnostics Development Unit, Department of Haematology, University of Cambridge, Cambridge, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Thomas</LastName><ForeName>Isabelle</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Curran</LastName><ForeName>Martin D</ForeName><Initials>MD</Initials></Author><Author ValidYN="Y"><LastName>Ellis</LastName><ForeName>Joanna S</ForeName><Initials>JS</Initials></Author><Author ValidYN="Y"><LastName>Parmar</LastName><ForeName>Surendra</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Goel</LastName><ForeName>Neha</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Sharma</LastName><ForeName>Pia I</ForeName><Initials>PI</Initials></Author><Author ValidYN="Y"><LastName>Allain</LastName><ForeName>Jean-Pierre</ForeName><Initials>JP</Initials></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Helen H</ForeName><Initials>HH</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><Acronym>WT_</Acronym><Agency>Wellcome Trust</Agency><Country>United Kingdom</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>07</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Clin Microbiol</MedlineTA><NlmUniqueID>7505564</NlmUniqueID><ISSNLinking>0095-1137</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001530" MajorTopicYN="N" Type="Geographic">Belgium</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009980" MajorTopicYN="N">Influenza A virus</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009981" MajorTopicYN="N">Influenza B virus</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007251" MajorTopicYN="N">Influenza, Human</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D025202" MajorTopicYN="N">Molecular Diagnostic Techniques</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009297" MajorTopicYN="N">Nasal Mucosa</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009305" MajorTopicYN="N">Nasopharynx</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010614" MajorTopicYN="N">Pharynx</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019095" MajorTopicYN="Y">Point-of-Care Systems</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006113" MajorTopicYN="N" Type="Geographic">United Kingdom</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014773" MajorTopicYN="N">Virology</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>7</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>7</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>3</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId 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l'Est</li></region><settlement><li>Cambridge</li></settlement><orgName><li>Université de Cambridge</li></orgName></list><tree><noCountry><name sortKey="Allain, Jean Pierre" sort="Allain, Jean Pierre" uniqKey="Allain J" first="Jean-Pierre" last="Allain">Jean-Pierre Allain</name><name sortKey="Curran, Martin D" sort="Curran, Martin D" uniqKey="Curran M" first="Martin D" last="Curran">Martin D. Curran</name><name sortKey="Ellis, Joanna S" sort="Ellis, Joanna S" uniqKey="Ellis J" first="Joanna S" last="Ellis">Joanna S. Ellis</name><name sortKey="Goel, Neha" sort="Goel, Neha" uniqKey="Goel N" first="Neha" last="Goel">Neha Goel</name><name sortKey="Lee, Helen H" sort="Lee, Helen H" uniqKey="Lee H" first="Helen H" last="Lee">Helen H. Lee</name><name sortKey="Parmar, Surendra" sort="Parmar, Surendra" uniqKey="Parmar S" first="Surendra" last="Parmar">Surendra Parmar</name><name sortKey="Sharma, Pia I" sort="Sharma, Pia I" uniqKey="Sharma P" first="Pia I" last="Sharma">Pia I. Sharma</name><name sortKey="Thomas, Isabelle" sort="Thomas, Isabelle" uniqKey="Thomas I" first="Isabelle" last="Thomas">Isabelle Thomas</name></noCountry><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Wu, Liang Ta" sort="Wu, Liang Ta" uniqKey="Wu L" first="Liang-Ta" last="Wu">Liang-Ta Wu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|wiki= Wicri/Sante
|area= GrippeBelgiqueV4
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|clé= pubmed:23850955
|texte= Duplex molecular assay intended for point-of-care diagnosis of influenza A/B virus infection.
}}
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